nc1 at ACPUB.DUKE.EDU
Fri Mar 14 12:13:19 EST 1997
I have wanted to make 2X agar (4%) solution, but every time I try I get
overflow after autoclaving. First I autoclaved agar in water for 20min
to make less than 500mL in 2-L erhlenmeyer flask, and I got an overflow.
Then, I melted agar with microwave before autoclave, and I got another.
Reduced the autoclave time to 15min, and I still got an overflow. What's
wrong? Do I miss something everyone else knows?
Rm. 213 MSRB, Box 3345
Program in Molecular Cancer Biology
Duke University Medical Center
Durham, NC 27710
nc1 at acpub.duke.edu
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