4% Agar?

[Namjin Chung]

Dear Yeasters,

I have wanted to make 2X agar (4%) solution, but every time I try I get 
overflow after autoclaving.  First I autoclaved agar in water for 20min 
to make less than 500mL in 2-L erhlenmeyer flask, and I got an overflow.  
Then, I melted agar with microwave before autoclave, and I got another.  
Reduced the autoclave time to 15min, and I still got an overflow.  What's 
wrong?  Do I miss something everyone else knows?  

Steamed 


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  Namjin Chung
  Rm. 213 MSRB, Box 3345
  Program in Molecular Cancer Biology
  Duke University Medical Center
  Durham, NC 27710
  nc1 at acpub.duke.edu

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