Are PCR reactions stable at 4 degrees?

Jan Hoolwerf hoolwerf at
Fri Mar 14 01:06:19 EST 1997


Harry Witchel <Harry.Witchel at Bristol.Ac.Uk> wrote:

 HW> and store it in the refrigerator for 3-4 days (as a PCR reaction,
 HW> with oil and all the reactants still in it, I can't do the second
 HW> half of the reaction (the result is usually a smear with no
 HW> indication of the correct band).

We always freeze (-20°C) the PCR reactions when not to be used the same day.
Seems to work. :-)

with regards, Jan Hoolwerf

******************* these words were mine, all mine !! ********************
 J.D. Hoolwerf
 Neth. Inst. for Dairy Research
 P.O. Box 20                        phone  : 31-318-659511
 6710 BA  Ede                       fax    : 31-318-650400
 the Netherlands                    e-mail : hoolwerf at

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