universal standard?

xudong huang xudong.huang at endo.mas.lu.se
Fri Mar 14 11:13:30 EST 1997


Hello,

I may have 200 to 400 human muscle genes of potential interest to study
their mRNA expression, i.e. genes recovered from diferential display.
Since the amount of RNA extracted from muscle biopsies is quite limited,
therefore the PCR quantitation will be the better choice than other
methods. The ideal thing is that there is a 'universal house keeping
gene' as the internal standard for all of those target genes and this
house keeping gene is not regulated by glucose and insulin. Is it
possible? Any suggestions and comments are welcome.Thanks.

Xudong Huang



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