Q: PCR cloning

Dimitri Harmegnies ldewit at ben.vub.ac.be
Fri Mar 14 09:37:12 EST 1997


Well, it is possible that your sites are too close to the end of your
fragments.
To have an efficient cut of EcoR I when this site is close to the end,
you have to have this sequence: CCGG GAATTC CGG
For BamHI, it is:CGC GGATCC GCG

Dimitri Harmegnies
ldewit at ben.vub.ac.be
Dept. of Virology
Institut Pasteur de Bruxelles
642 rue Engeland
B-1180
Brussels, Belgium



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