western blot

S K Marquardt smarquardt at earthlink.net
Sat Mar 15 19:35:18 EST 1997


David Tak Yan Yu wrote:
> 
> I have been trying to do western blot with antibodies against immediate
> early genes eg c-fos.  There is quite a lot of background.  I would
> appreciate if advices.
> 
> David Yu
> --
> 35-40 Rehab Center,  Rheumatology Division, UCLA, Los Angeles,
> CA90095-167022.
> Tel: (310)-395-5909, FAX: (310)-206-8606.
> E-mail: dyu at med1.medsch.ucla.edu  or dtyyu at ucla.edu
I've seen greatly enhanced signal with TBS over PBS in AP systems. 
Also, in my hands, Blotto and Gelatin are superior for blocking over BSA
and tween, etc.  Also it helps to preincubate primary and secondary
antibodies in a buffer with your blocking agent.

s marquardt



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