Membrane protein prep

Shinhan Shiu sshiu at
Sat Mar 15 17:35:57 EST 1997

Dear bionetters,

I am trying to purify a plant transmembrane receptor. When I go through the 
literature, some groups advise aganist the use of boiling in loading buffer 
prior SDS-PAGE for membrane protein. 

I wonder if you have experience with membrane protein and found this to be 


Shinhan Shiu
Department of Botany University of Wisconsin-Madison
132 Birge 430 Lincoln Dr, Madison, WI 53706
tel: 608-262-4008
sshiu at

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