Membrane protein prep

[Shinhan Shiu]

Dear bionetters,

I am trying to purify a plant transmembrane receptor. When I go through the 
literature, some groups advise aganist the use of boiling in loading buffer 
prior SDS-PAGE for membrane protein. 

I wonder if you have experience with membrane protein and found this to be 
true.

 


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Shinhan Shiu
Department of Botany University of Wisconsin-Madison
132 Birge 430 Lincoln Dr, Madison, WI 53706
tel: 608-262-4008
sshiu at students.wisc.edu
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