Effect of NaOH on biotin-streptavidin??

Gordon Munro g_munro at globalnet.co.uk
Sat Mar 15 11:21:52 EST 1997


A question: I know that NaOH affects the binding between biotin and the
nucleic acid it is bound to (the OH- attacks the amide bonding in the
linker molecule between the biotin and nucleic acid). Does anyone have
an idea on what the relation between NaOH concentration and the breakage
of the biotin-nucleic acid bonding is ? E.g. does 1mM NaOH still have a
significant effect ?




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