RNase-free Glycogen Solution

brett brett at BORCIM.WUSTL.EDU
Sun Mar 16 16:59:04 EST 1997


>Dear Netters,
>
>I want to make RNase-free glycogen solution (to ppt RNA).  Could someone there 
>give me some idea?
>
>Thanks in advance
>Xiao-yu Liu
>
>
>
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I find Boehringer's glycogen solution to work well for this, and haven't found
it to have any RNases. If you're isolating glycogen from tissue or even using
dry reagents, you may just want to treat it with ProteinaseK/SDS and/or phenol
extracting/ethanol precipitating it. 


Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             




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