FISH probe: random prime or nick translate?

W R Bennett bspwrb at
Mon Mar 17 10:08:19 EST 1997

Are there any good reasons not to use a random priming method to generate 
biotinylated or dig-labelled probes for FISH?  The protocols I have all 
use nick translation, but - at least in Southerns - we've had a lot more 
success with random primed probes than with nick translation.


Bill Bennett

More information about the Methods mailing list