FISH probe: random prime or nick translate?

W R Bennett bspwrb at bath.ac.uk
Mon Mar 17 10:08:19 EST 1997


Are there any good reasons not to use a random priming method to generate 
biotinylated or dig-labelled probes for FISH?  The protocols I have all 
use nick translation, but - at least in Southerns - we've had a lot more 
success with random primed probes than with nick translation.

Thanks,

Bill Bennett
W.R.BENNETT at bath.ac.uk




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