NP-40 Life?
Wolfgang Schechinger
wgschech at med.uni-tuebingen.de
Mon Mar 17 07:35:01 EST 1997
That will depend on your contaminants!
When using solns stored for a longer time, it's good to have a
critical look on possible forms of extraterrestrial life (slimy
streaks and so on) inside your bottle. In any case of doubt, throw
the contents away and start over. If the content is precious, add a
little to antibiotic free (cell) culture medium and incubate
overnight. If there are some buggers inside, they will grow.
Sometimes it may be good to aliquot your solns and/or freeze them
down. If this is not possible, consider addition of preservatives as
sodium azide, thimerosal, etc.
Good luck!
Wolfgang
> > What's a typical lifetime for a solution of 10% NP-40
> > that's stored at 4 degrees?(the fridge)
> >
> IMHO that is stored for very long time.
> Alex
>
>
---------!all possible disclaimers apply!-----------
Wolfgang Schechinger
University of Tuebingen
email: wgschech at med.uni-tuebingen.de
http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
public PGP key is avilable on request
More information about the Methods
mailing list