western blot

[mfm### at rzmail.uni-erlangen.de]

Rupert Yip wrote:
 
> Is there any reason why TBS is used over PBS?  Is one superior to the
> other?

Yes, if you use anti-phosphotyrosine, -serine or -threonin antibodies, 
PBS (PHOSPHATE-buffered saline) is not recommended to use, but TBS works 
fine. And to have only one buffer system used, we allways use TBS.

bye
	Heinz
-- 
Heinz-Juergen Schaefers
University of Erlangen-Nuernberg
Dept. of Medicine IV
Nephrology Research Lab
h-j.schaefers at popHost.com