Whole genome amplification?

Yvon CAVALOC cavaloc at sun-recomgen.univ-rennes1.fr
Mon Mar 17 05:32:18 EST 1997


I am currently trying to amplify the whole human genome starting with
very low amounts of DNA (< 5pg) using a degenerate PCR strategy. Since I
cannot amplify more than 200 fold the genome, I would like to find
people experienced in this topic. Has anybody been able to obtain a
strong general amplification with a totally degenrated primer (N15, N10,
N6?) or is it better to use degenerated primer having fixed 5' sequence?
Thank you for help

Yvon CAVALOC
UPR 41 CNRS
Faculte de Medecine
2, Av du Pr L Bernard
35043 RENNES cedex - France

cavaloc at sun-recomgen.univ-rennes1.fr



More information about the Methods mailing list