organic P determination..

[Frank R. Gorga]

ANDY JOHNSTON wrote:
> 
> Hi,
> 
> I am looking for some help on converting organic P into inorganic PO4 so I can
> use the ascorbic acid Mo assay to measure it.
> 
> Anyone tell me how to convert one to the other?
> 
> Many thanks
> 
> Andrew Johnston
> Dundee

I use 9:1 (v/v) conc. H2SO4: 72% HClO4 when assaying for phospholipids.
I add 200 microliters of acid to a dried organic residue and heat at 180
oC until the samples are clear, usually about 1 h.

Hope that this helps,

-- Frank 

***********************************
Frank R. Gorga, Ph.D.
Dept. of Chemistry & Biochemistry
Duquesne University
Pittsburgh, PA 15282
412-396-5858 / 412-396-5683 (fax)
gorga at next.duq.edu