Shaun D. Black
shaun at uthct.edu
Mon Mar 17 12:02:18 EST 1997
rollins.24 at postbox.acs.ohio-state.edu (Sean Rollins) wrote:
> I'm trying to synthesize 4 thio UTP and I'm losing my sample at the
>4 thio UDP in 100ul and the phosphate is transfered. This is brought up to
>volume of 1:1 ethanol/acetone. I've checked my reaction by HPLC and I'm
>losing my sample at the precipitation step. No details are given on this
>precipitation step (time, temp and spin conditions). If anyone has any >advice 1 or any 4 thio UTP for a possitive control it would be appreciated.
A while back, we found that we could get very small oligos to precipitate
if we simply put them in the -20C freezer for a couple of hours (of course,
after ethanol precipitation). I'm not sure this is the answer to your problem, but perhaps it is worth consideration or a try.
= Shaun D. Black, PhD | Internet address: shaun at uthct.edu =
= Dept. of Biochemistry | University of Texas Health Center, at Tyler =
= World Wide Web: http://pegasus.uthct.edu/UTHCT-Home/Welcome.html =
More information about the Methods