gel shift assay

[John Watson]

Joachim Kremerskothen wrote:


> we have some problems with our gel shift assays because our protein/RNA
> complex do not run on a native 5% polyacrylamide gel using TBE buffer (pH
> 7,6). Any suggestions for another gels system, etc.?

My feeling is that the gel and buffer system can be awfully protein-specific.  
Back in the days when I was doing gel-shift, I got the best results for my 
protein (the dioxin receptor) using 20mM Hepes-20 mM Tris-1 mM EDTA-6% PA 
(19:1 acrylamide:bis-acrylamide) gels (pH 8).

AJW
----------------
John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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"If you're not part of the solution, you're part of the precipitate."
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