electroporation of primary spleen cells

Will Fuller wf10000 at cus.cam.ac.uk
Mon Mar 17 10:42:54 EST 1997


dear all

I am trying to electroporate primary spleen cells from BalbC mice and I'm having real problems getting the cells transfected.  I'm using the BioRad gene pulser.  Has anyone else had any experience in these cells and if so c
 could they give me any advice or helpful hints                                     
 Could you please reply to wf10000 at cus.cam.ac.uk

 thanks          
 
 Will                                                       n



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