troubles with antibiotic

Namy olivier onamy at
Tue Mar 18 02:18:20 EST 1997

I am a new student in bacteriology, and I  would like to know something:
- Why, when I use the antibiotic spectinomycine to select a
transformation of gram + bacterium, I obtain too many spontaneus
colonies (bacteria whithout the vector, but spcR!)?.
-Does anybody know how act this antibiotic? and which mecanism(s?) allow
bacteria to become resistant?
Many thanks for your help,
Olivier.  (France)

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