Q: SAP (Shrimp alkaline phosphatase) at 4degC

[Hiranya Roychowdhury]

At 09:51 AM 3/18/97 -0000, Wolfgang Schechinger wrote:
>Dear netters,
>
>By accident, I stored Boehringer Mannheim's SAP at 4degC (instead 
>-20degC as it should be) for three month. Does it make sense to run 
>activity tests or should I just throw it away?
>
>Thanks for all comments!
>
>Wolfgang
>---------!all possible disclaimers apply!-----------
>                                     
>Wolfgang Schechinger
>University of Tuebingen
>email: wgschech at med.uni-tuebingen.de
>
>http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
>
>public PGP key is avilable on request	
>
>

My experience with the SAP has been that, over time, much of its activity is lost even when stored at the bottom of the -20 C freezer. I suspect that the act of taking the tube out as and when needed, coupled with the fact that my enzyme box has other enzymes that I use every few weeks, is sufficient to gradually do the SAP in. I will have to aliquot working amounts even more stringently! 

... So my guess would be that your enzyme has kicked the bucket by now.

Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu