293 cells with transformed phenotype?

[Ian A. York]

In article <graham-1803971207460001 at tastiger.dbe.csiro.au>,
Lloyd Graham <graham at dbe.csiro.au> wrote:
>At ATCC+12 passages, my 293 cells (HEK293) have spontaneously changed
>phenotype to poorly adhered cells that grow in clumps, rather as if they
>have undergone a spontaneous transformation. All I did was subculture them

Have you checked for mycoplasma?  I've not seen this happen with any of
the 293 I've carried.

Ian
-- 
      Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
      "-but as he was a York, I am rather inclined to suppose him a
       very respectable Man." -Jane Austen, The History of England