SDS effect ?

jing at NIC.HBU.EDU.CN jing at NIC.HBU.EDU.CN
Tue Mar 18 08:51:11 EST 1997

Hellow bionetters,

We isolated a glycoprotein and identified by Dot blotting with Dig 
glycan/protein double labeling kit from Boehringer Mannheim. Why could only 
protein bands be stained but no sugar bands appeared on NC after Western 
blotting,can SDS break the linkage between protein and sugar?
Many thanks for your help!

X.Y. Zhao

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