western blot

Alix Helme-Guizon ahelme at lovelace.infobiogen.fr
Tue Mar 18 07:11:23 EST 1997

> Is there any reason why TBS is used over PBS?  Is one superior to the
> other? 
some people says (but maybe without any good reason) that PBS reduce the
activity of the alcaline phosphatase. i didn't notice that myself, but it
seems to me that my westerns worked better with TBS-tween. and you can
recycle the antibody in TBS for further re-use, and this is a good point!

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