detection of proteins in FPLC

Achim Recktenwald, PhD achim at ibex.ca
Tue Mar 18 17:28:59 EST 1997


ITABAJARA_DA_SILVA_VAZ_JU,ita at DNA.CBIOT.UFRGS.BR@invivo.edu wrote:
> 
> Please,
> Is there a simple method of transform the AU detected by  monitor UV in FPLC
> in mg/ml concentration?


Only if you know the molar extinction coeffcient of your protein. Since
every protein has its specific value you are out of luck. 
If you search for a list of many proteins, check out:
G.D. Fasman, CRC Practical Handbook of Biochemistry and Molecular
Biology, 1990, pp.196-358.

Additionally, this only works, if you do not have any other absorbing
components in your elution-peak, like nucleic acids.

Achim

__________________________
Achim Recktenwald, PhD.
IBEX Technologies, Inc.
Biochemistry
5485 rue Pare
Montreal, PQ, H4P 1P7
Canada

achim at ibex.ca



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