Membrane protein prep

Dr. David J. Meyer meyerdj at phibred.com
Wed Mar 19 17:20:44 EST 1997


Hello,

Yes, for some proteins this can cause aberrant migration. I used to
see fuzzy bands for the tomato H+-ATPase if samples were boiled, but
sharp bands if heated for a longer time at lower temperatures (with
added protease inhibitors).

-DJ





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