detection of proteins in FPLC
bipin k. dalmia
dalmiabk at phibred.com
Wed Mar 19 15:53:07 EST 1997
On Tue, 18 Mar 1997 21:49:38 GMT, bspwrb at bath.ac.uk (W R Bennett)
>Assuming 280nm detection, 1.00 AU is roughly equivalent to 1.0 mg/ml, so,
>for example, if your sensitivity is set to 0.01, then full scale
>deflection is roughly 10ug/ml.
this would assume a flow-cell path length of 1.0 cm and i do not think
that detector flow-cells have a 1.0 cm path length.
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