detection of proteins in FPLC

bipin k. dalmia dalmiabk at
Wed Mar 19 15:53:07 EST 1997

On Tue, 18 Mar 1997 21:49:38 GMT, bspwrb at (W R Bennett)

>Assuming 280nm detection, 1.00 AU is roughly equivalent to 1.0 mg/ml, so,
>for example, if your sensitivity is set to 0.01, then full scale
>deflection is roughly 10ug/ml. 

this would assume a flow-cell path length of 1.0 cm and i do not think
that detector flow-cells have a 1.0 cm path length.


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