Sterilisation of Bead-coupled antibodies
odiettri at ich.ucl.ac.uk
Tue Mar 18 14:42:00 EST 1997
I want to use antibody covered acrylic resin beads in sterile cell
culture. Autoclaving and filtering is not possible.
Could I simply add some Na-azide, incubate for hours, days, etc. and wash
the beads subsequently with sterile medium? Or would there be still some
nasty spores which ignored the azide and began to grow as soon as it is
If so, do you know of any other sterilisation method that wouldn't affect
my antibodies but kill the germs?
Thanks in advance for any ideas,
Postdoctoral Research Assistant
Institute of Child Health
University College of London
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