hot glass plates from acrylamide gels
salger at wap1.zi.biologie.uni-muenchen.de
Wed Mar 19 18:56:48 EST 1997
Timothy R Jackson (tjackson at uoguelph.ca) wrote:
: has anybody experienced the following problem and managed to solve it...
: we are using 6% denaturing acrylamide gels to separate pcr amplified
: microsatellites. one of our primers are end-labelled with gamma
: 33P-ATP. after running our gels, we fix them in a 10% methanol, 10%
: acetic acid solution and then dry them. we have recently found that when
: we remove our gel from fixer and lift it off of the glass plate, the
: glass plate that the gel was fixing on is radioactive in the same region
: as the amplified microsatellite locus. in some cases the glass plate is
: hotter than the gel!! this appears to be affecting the consistancy of
: our results as some glass plates get hotter than others and could be
: reducing the signal of our gel. any solutions????
I experienced the same problem once when doing gelshifts. Cleaning the
plates VERY thoroughly with ethanol and water followed by siliconization
fixed the problem for me.
Klaus Salger phone : +49 (0)89 5902 -502
Zoologisches Institut FAX : -450
AG Wetterauer e-mail: salger at zi.biologie.uni-muenchen.de
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