E. coli strain MC1061/p3

[John Richard Seavitt]

On 18 Mar 1997, Woong Choi,M.D. wrote:

> Is there anybody who can provide me an E.coli strain MC1061/p3.
> I need MC1061/p3 for pCDM8 plasmid amplification.


MC1061/p3 can be obtained from Invitrogen.  The P3 episome has a tendency
to undergo reversions at the amber mutations within the amp and tet
resistance genes (especially when cultured in excess amp/tet).

John Seavitt