nested-PCR?

Huang Ke-xue khuang at CHEMVX.CHEM.TAMU.EDU
Thu Mar 20 14:45:51 EST 1997


At 12:41 AM 3/20/97 -0800, you wrote:
>Dear neter:
>I want to know what means nested PCR and its real application. Thanks in 
>advance
>Ma

Hi, there,
As I know, netsed PCR means first you amplify the gene by using two primers,
then you use this PCR product as template and do the secod PCR by using one
or two inside primers (inside the first two primers). 
I have used it for cloning gene by design four primers based on the
homologous of other cloned gene, and RACE.

Hope this help!


Huang   




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