Screening lambda gt11 library

Hiranya Roychowdhury hroychow at NMSU.EDU
Thu Mar 20 17:38:04 EST 1997


At 08:48 PM 3/20/97 GMT,  wrote:
>I am interested in screening a lambda library with antibodies.  Rather than use
>an 125 Iodine labeled secondary (anti-rabbit), I was considering using 35s 
>labeled protein A.  Does anybody out there have any experience with this?  
>Is this a reasonable substitute for 125I? 
>		
>                Todd Lane
>
>

Why would you want to do RIA for screening the library? What's wrong with the chromogenic detection?

Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu



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