Nylon Membrane Troubles

Michael J. Prigge prigge at darkwing.uoregon.edu
Thu Mar 20 17:05:37 EST 1997


griffcouch at aol.com (GriffCouch) wrote:

> I am having a problem with DNA and RNA going THROUGH the nylon membrane I
> am trying to transfer to. The nucleic acids are well above the 300 bp cut
> off that the manufacturer recommends for the 0.45 pore size. I am awaiting
> a shipment of 0.2 pore membrane to test it anyway. Can anyone suggest
> anything else? All other conditions, solutions, etc., have remained the
> same. I have tried both capillary and vacuum transfer.
> 
> Maureen S. Wright, Ph.D.
> US Dept. of Agriculture

Are you using un-charged or pos. charged nylon. The lab down the hall says
that they found the same phenomenon with uncharged. They switched to
magnacharge membrane and it took care of the problem. 
Mike
Mike Prigge                    prigge at darkwing.uoregon.edu
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