ppt. Small RNA

daniel jacobs d.jacobs at unsw.edu.au
Tue Mar 18 02:40:44 EST 1997


In article <33268C12.55D8 at molbio.uoregon.edu>, Matt Thomas
<Thomas at molbio.uoregon.edu> wrote:

> I have this crazy idea for an experiment where I need to recover the
> products of an RNase T1 digest.  What I want to know is, how easy is it
> to Etoh ppt. RNA oligos on the range of 7-11mers?  I'm planing to try it
> with NaoAct and EtoH like any normal ppt but I also plan to throw in
> glycogen for carrier.  If anyone has any experience or suggestions
> please e-mail me.  
> 

Why not give 9 volumes of butanol a try i.e. like a primer preciptation. I
dont know if it will work but on the other hand it won't take long to find
out.

daniel



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