QE: nuclear extract

Dr.Tilman Voss voss at bender.co.at
Thu Mar 20 14:35:38 EST 1997

Lloyd Graham wrote:
> At ATCC+12 passages, my 293 cells (HEK293) have spontaneously changed
> phenotype to poorly adhered cells that grow in clumps, rather as if they
> have undergone a spontaneous transformation. All I did was subculture them
> in a standard way.
> I have just made a series of stable 293 transfectants (starting with cells
What is the best method for the extraction of nuclear proteins.
I want to separate these proteins on 2D gels.
Tilman Voss
Boehringer-Ingelheim Research Vienna

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