How to clone a 20 bp fragment?

Yvon CAVALOC cavaloc at sun-recomgen.univ-rennes1.fr
Thu Mar 20 05:59:22 EST 1997


Hi Karin,

what I did usually and that worked perfectly was to clone synthetic
oligonucleotides that I had annealed before. Let me know if you are
interested in a detailed protocol taht gave me 100% efficiency (a 30 bp
in a 3 kb vector)

Bye,
Yvon



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