nested-PCR?

Yvon CAVALOC cavaloc at sun-recomgen.univ-rennes1.fr
Thu Mar 20 05:30:45 EST 1997


Hello,
a nested PCR is used to amplify week amounts of DNA. You first perform a
PCR with external oligos and you use the resulting matrix to perform a
second PCR with internal oligo.

First PCR:
		-->
--------------------------------------------------------------------
--------------------------------------------------------------------
									        		<--


Second PCR:
					-->
		------------------------------------------------
		------------------------------------------------
										<--

"The two last oligos are located in a NEST"

Yvon



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