agarose gel electrophoresis

Paul N Hengen pnh at ncifcrf.gov
Fri Mar 21 13:43:15 EST 1997


Andreas Rump (arump at imb-jena.de) wrote:

> I have observed the following phenomenon several times: when I cut 
> out a piece of agarose gel containing a single DNA band and place this
> piece of gel on it's side, then I see two bands. This means, that the
> same DNA moves through the gel on two different levels (x and y, see
> Figure), one near the bottom of the gel and one near the surface. 
> Both bands are discrete, although the slot has been completely filled
> with sample and one would expect that the DNA enters the gel at the
> whole heigth of the gel and would move as one broad band.

I have seen it too. Do you use a tracking dye that migrates faster than
the DNA front? Does the same separation happen if you exclude the dye?

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