ligation and UV
zhanglin at CCO.CALTECH.EDU
Fri Mar 21 13:29:12 EST 1997
Hi. A recent Biotechniques article describes a means
to improve ligation/transformation efficiency by using
Guanosine to protect DNA during gel purification.
See Biotechnique 21 (5) 898-903 (1996). Have any of
you tried this? or would you comment on this seemingly
simple method? If you have tried, then could you
give me an idea as to what kind of improvement one
might get when 366-nm UV irradiation is used (instead
of 312 nm)?
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