BSA in PCR reaction: role of?
Dr. Duncan Clark
duncan at genesys.demon.co.uk
Fri Mar 21 04:03:22 EST 1997
In article <9702218589.AA858984377 at smtpgwy.agric.nsw.gov.au>, Deborah.Ha
ilstones at SMTPGWY.AGRIC.NSW.GOV.AU writes
> what is the role of BSA whan added to a PCR reaction? It is reported
> to improve the results, but is this by providing non-specific targets
> for proteases or binding (proteinaceous) inhibitors or what? any ideas
> or experiences appreciated.
Most PCR's use 2.5 u of Taq which is only 10ng of protein (sp. act. of
Taq is 250,000u/mg). Addition of BSA increases the protein concn. and is
of value if using say the Idaho hot air PCR machine which uses glass
capillaries. It reduces the amount of enzyme you lose through non-
specific binding to plastic or glass tubes.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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