kraev at bc.biol.ethz.ch
Fri Mar 21 08:44:07 EST 1997
In article <5grpjp$bhg at eldborg.rhi.hi.is>, thorarb at rhi.hi.is (Thorarinn
> I'm curious about quikchange mutagenesis kit from stratagene.
> has anyone experince concerning the kit? Is it as fast and accurate
> as advertized? What is the max size of plasmid it can mutate?
In our lab many people have used a similar kit from Pharmacia (aka Stratagene's
Chamaleon), and results range from excellent to unusable. The reason for that
I think is in the design of the mutagenic primer. Sometimes, it was
by eyeballing. Sometimes, it was designed by a program without sufficient
blended in, and apparently did not anneal, which could later be traced to
structure potential at the site of annealing. However, the available
not point to a clear set of rules. As you always have to gamble with the
the companies can use it to make "sureshot" kits, which do work with the
control included, but no one can sue a company for his bad luck.
I hope this helps,
Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
e-mail kraev at bc.biol.ethz.ch
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