native e. coli lysis/ inexpensive sonicator supplier source?

Steve Brown sbrown at chugaibio.com
Fri Mar 21 14:06:19 EST 1997


We are looking for a method of e. coli lysis that does not use =
lysozyme/denature proteins.  Three freeze/thaw cycles on dry ice /37 C =
yielded much less total protein than I saw using lysozyme.
I would like to try sonication but they are pretty expensive (~$1600).  =
Can anyone recommend a moderately priced sonicator? And/or another =
method for native lysis?
Thanks!




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