preferential amplification by proofreading Taq ???
dholst6016 at aol.com
Sat Mar 22 00:53:28 EST 1997
It is strange that you have problems amplifying the same size product with
Taq and proofreading Taq. One would suspect nonspecific priming. One
thought my be that since Taq polymerase has such a broad activity range,
30-85C, nonspecific priming may be an answer. Possible solutions may be
hot start PCR or use af a proofreading enzyme such as Pfu which has a much
tighter range of activity, hence less nonspecific product.
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