Wanted: 18S or 28S probe

Bernard Murray bernard at elsie.nci.nih.gov
Sat Mar 22 00:29:16 EST 1997

In article <3332AB1B.5719 at le.ac.uk>, ac50 at le.ac.uk says...
>Edmundo Castro wrote:
>> Hello all!
>You don't say what you want it for exactly. Hybaid (Shandon Lipshaw in
>the States) sell a 28S ribosomal RNA probe for use with in situ
>hybridisation studies.
>Hope this is of use

>> Our lab would like to obtain either an 18s or 28S probe, preferably
>> human.
>> Can anyone provide us with some or perhaps suggest a place where we can
>> purchase it?
>> Thank you to all in advance!
>> EC

I've used a probe for human 18S rRNA from ATCC (#77242) which is
a 1.5 kb piece in pBluescript.  Northerns of total RNA look very
nice when probed with this.

Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD, USA)

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