Help gels cracking
Marieke R. Koedood Zhao
rkoedood at bio.bu.edu
Fri Mar 21 11:54:00 EST 1997
re-hydrating my gels worked for me (most of the time) - either in water,
or better in glycerol.
But even better than vacuum-drying was drying my gels between sheets of
cellophame (again after re-hydrating my gel).
Kevin Gilbride (gilbride at bu.edu) wrote:
: In article <gruther-1803971712480001 at macg203o.bio.purdue.edu>,
: gruther at bilbo.bio.purdue.edu (George Rutherford) wrote:
: > In article <5glurs$opt at mercury.hgmp.mrc.ac.uk>, gmorley at hgmp.mrc.ac.uk
: > (Mr. G. Morley) wrote:
: > > A colleague of mine is having problems running her
: > >
: > > protein gels.
: Try lowering your TEMED and AP concentratations and/or degas.
: KJ. Gilbride
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