Transfection of mammalian cells

Tori D. Williams twilliam at BIOMED.MED.YALE.EDU
Fri Mar 21 15:06:33 EST 1997


Hello,

I was wondering if anyone had any opinions regarding the efficency of 
lipofectamine mediated transfection versus electroporation versus calcium 
phosphate.

I am using lipofectamine at the moment so if anyone has any suggestions 
on increasing efficency. I'd appreciate those too. I'm using AKR-2B 
cells, a mouse fibroblast line.

Thanks,
Tori




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