How to get rid of extra A added by Taq
Xinyu X Song
xsong at uoguelph.ca
Sat Mar 22 16:35:12 EST 1997
I am doing two steps PCR mutengenesis by using homemade Taq Polymerase.
Every time when I cloned my fragment and sequenced, I always found there
is an extra A mismatch in the sequences which is added from the first
step PCR by Taq at 3' end of that strand. In my situation, there is no
other enzyme available. Could anyone give some suggestion to get rid of
this extra A in first step PCR? My e-mail is : xsong at uoguelph.ca.Your help
will be really appreciated.
Thank you in advance.
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