DNA Auto-sequencing

Tom Chappell t.chappell at ucl.ac.uk
Fri Mar 21 10:53:17 EST 1997

In article <allen-2103972040160001 at>, allen at bc.ntu.edu.tw
(Ao-lin Hsu) wrote:

>Hello everyone:
>    I am facing a problem that is I bought a ABI kit(Dye-Terminator)
>yesterday but I lost the kit's buffer. Is there anyone know how to prepare
>the reaction buffer of this kit and give me the formula!
>    My machine is ABI373 and the name of the ABI Prism kit is "Dye
>Terminator Cycle Sequencing Core Kit" for DNA sequencing.
> By the way, could I buy the AmpliTaqDNA Polymerase, FS alone?
>thanks for all of you!

The ABI Prism Dye Terminator Kit that we use is an all-in-one system. If
you've lost the tube with the buffer in it, you've lost the Dye
Terminators and the polymerase. If this is the case with your kit, the
unfortunate solution is to buy another (expensive) kit...

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