Screening lambda gt11 library

Mauricio Neira cmneira at facstaff.wisc.edu
Sun Mar 23 16:02:41 EST 1997


Hi!

Are you wondering about sensitivity?, I screened a library once using
the chemoluminiscent system with HRP-labeled secondary antibodies,
You can use biotynilated as well.
I think you get  high sensitivity and you can also easily re-screen
your membranes.
Of course you have to coordinate adding the substrate solution and
wrapping the films, initially seems difficult, but actually not very
much...

Mauricio

On 20 Mar 1997 20:48:51 GMT, TLANE at watson.princeton.edu () wrote:

>I am interested in screening a lambda library with antibodies.  Rather than use
>an 125 Iodine labeled secondary (anti-rabbit), I was considering using 35s 
>labeled protein A.  Does anybody out there have any experience with this?  
>Is this a reasonable substitute for 125I? 
>		
>                Todd Lane




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