In vitro transcription/translation

Bresagen bresagen2 at adelaide.on.net
Mon Mar 24 04:41:40 EST 1997


Dear All,

We want to test our bacterial expression constructs with an in vitro
transcription/.translation kit. However all of the commercial kits we
have seen are designed for T7, T3 or SP6 RNA Polymerase and we want to
test the whole construct, not put our insert into another vector to
test. 

Does anyone know of a commercially available kit that uses E coli RNA
polymerase.

TIA
Rick Tearle



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