plamid screening

[Ed Marsden]

Srikumar_M._Raja,raja at uvic.ca@invivo.edu wrote:
> 
> I am very grateful if someone can supply me a protocol (not the one from
> promega) for screening recombinant plasmids based on their mobility
> differences on agarose gel.
> 
> Thanks in advance.
> DzungDiep
> 
> My address: dbdiep at uvic.ca
> 
>>>>>>>>>>>Try "A practical guide to molecular cloning 2nd edition" by Bernard Perbal (1988). John Wiley & Sons.
This reference holds a miniscreen method based on the Birnboim & Doly 
approach along with screening on nitrocellulose, direct analysis on 
agarose etc...
							-Neil