How to get rid of extra A added by Taq
cmneira at facstaff.wisc.edu
Sun Mar 23 16:25:09 EST 1997
Maybe you want to listen a smart answer, as add this and you will
inhibit this specific activity of the enzyme...
I would not even try to GET RID OFF any extra As...but GET RID OFF
your boss who does not buy another enzyme for you!! :-)
Just black humor!,
On 22 Mar 1997 21:35:12 GMT, xsong at uoguelph.ca (Xinyu X Song) wrote:
>I am doing two steps PCR mutengenesis by using homemade Taq Polymerase.
>Every time when I cloned my fragment and sequenced, I always found there
>is an extra A mismatch in the sequences which is added from the first
>step PCR by Taq at 3' end of that strand. In my situation, there is no
>other enzyme available. Could anyone give some suggestion to get rid of
>this extra A in first step PCR? My e-mail is : xsong at uoguelph.ca.Your help
>will be really appreciated.
>Thank you in advance.
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