Running DNA on acrylamide gel

Rani Maurice Geha geha at hsc.usc.edu
Mon Mar 24 16:32:00 EST 1997


Is anybody aware of an equation or any other method that determines to 
DNA migration distance on acrylamide gel if all the parameters are 
known?

For example if you know the acrylamide:bisacrylamide ratio and 
concentration, the amperage, the wattage, the voltage and the 
temperature of the run, and you also know the size of the DNA fragment 
and the running time. Can you calculate the distance migrated?

Thanks in advance

Rani.



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